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Top of pageRESULTSTargeting of the murine Pkhd1 geneWe targeted exon 2 of the mouse Pkhd1 gene with a conventional LoxP flanked phosphoglycerate kinase (PGK) neomycin cassette (Figure 1a) and screened by Southern blotting (Figure 1b). Mice were derived from three separate embryonic stem cell clones to generate Pkhd1neo2 animals and these